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人輪狀病毒抗原酶免試劑盒,(RV Ag)ELISA檢測試劑盒,英文名:RV Ag ELISA Kit,英文縮寫:RV Ag ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人輪狀病毒抗原ELISA檢測試劑盒,RV Ag 檢測試劑盒,RV Ag 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人輪狀病毒抗原酶免試劑盒,(RV Ag)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
核因子κB2(NFκB2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Kappa B2 (NFkB2)48T/96TY10035Mus musculus (Mouse)
V-Rel網(wǎng)狀內(nèi)皮增生病毒癌基因同源物B(RELB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB)48T/96TY10036Mus musculus (Mouse)
軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Netrin 1 (Ntn1)48T/96TY10037Homo sapiens (Human)
軸突生長誘向因子4(Ntn4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Netrin 4 (Ntn4)48T/96TY10038Homo sapiens (Human)
載脂蛋白A4(APOA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein A4 (APOA4)48T/96TY10039Homo sapiens (Human)
E選擇素(SELE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selectin, Endothelium (SELE)48T/96TY10040Sus scrofa; Porcine (Pig)
解整合素金屬蛋白酶9(ADAM9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 9 (ADAM9)48T/96TY10041Homo sapiens (Human)
解整合素金屬蛋白酶9(ADAM9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 9 (ADAM9)48T/96TY10042Rattus norvegicus (Rat)
解整合素金屬蛋白酶9(ADAM9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 9 (ADAM9)48T/96TY10043Mus musculus (Mouse)
組織轉(zhuǎn)谷氨酰胺酶(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transglutaminase 2, Tissue (TGM2)48T/96TY10044Homo sapiens (Human)
音猬因子(SHH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hedgehog Homolog, Sonic (SHH)48T/96TY10045Homo sapiens (Human)
白介素12(IL12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 12 (IL12)48T/96TY10046Homo sapiens (Human)
補體因子D(CFD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Factor D (CFD)48T/96TY10047Homo sapiens (Human)
毒蕈堿型膽堿受體M1(CHRM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 1 (CHRM1)48T/96TY10048Homo sapiens (Human)
白介素10(IL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 (IL10)48T/96TY10049Bos taurus; Bovine (Cattle)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY10050Bos taurus; Bovine (Cattle)
凝血因子Ⅺ(F11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XI (F11)48T/96TY10051Homo sapiens (Human)
絲切蛋白2(CFL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cofilin 2, Muscle (CFL2)48T/96TY10052Homo sapiens (Human)
紐蛋白(VCL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vinculin (VCL)48T/96TY10053Homo sapiens (Human)
極低密度脂蛋白(VLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Very Low Density Lipoprotein (VLDL)48T/96TY10054Chicken (Gallus)
核因子κB抑制因子α(IκBα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)48T/96TY10055Rattus norvegicus (Rat)
核因子κB抑制因子α(IκBα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)48T/96TY10056Homo sapiens (Human)
血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serum Amyloid A (SAA)48T/96TY10057Oryctolagus cuniculus (Rabbit)
結(jié)合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Haptoglobin (Hpt)48T/96TY10058Oryctolagus cuniculus (Rabbit)
白介素13(IL13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 13 (IL13)48T/96TY10059Sus scrofa; Porcine (Pig)
核因子κB抑制因子β(IκBβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibitory Subunit Of NF Kappa B Beta (IkBb)48T/96TY10060Homo sapiens (Human)
核因子κB(NFκB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Kappa B (NFkB)48T/96TY10061Rattus norvegicus (Rat)
核因子κB抑制因子β(IκBβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibitory Subunit Of NF Kappa B Beta (IkBb)48T/96TY10062Rattus norvegicus (Rat)
β-1,4-半乳糖轉(zhuǎn)移酶1(β4GALT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-1,4-Galactosyltransferase 1 (b4GALT1)48T/96TY10063Homo sapiens (Human)
Ⅰ型膠原交聯(lián)羧基端肽(CTXⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cross Linked C-opeptide Of Type I Collagen (CTXI)48T/96TY10064Rattus norvegicus (Rat)
黑素瘤細(xì)胞黏附分子(MCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melanoma Cell Adhesion Molecule (MCAM)48T/96TY10065Canis familiaris; Canine (Dog)
血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY10066Homo sapiens (Human)
含Rho關(guān)聯(lián)卷曲螺旋蛋白激酶2(Rock2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2)48T/96TY10067Homo sapiens (Human)
AMP激活蛋白激酶β1(PRKAβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase, AMP Activated Beta 1 (PRKAb1)48T/96TY10068Danio rerio (Zebrafish)
硝基酪氨酸(NT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitrotyrosine (NT)48T/96TY10069General
谷胱甘肽過氧化酶1(GPX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione Peroxidase 1 (GPX1)48T/96TY10070Rattus norvegicus (Rat)
類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tryptase (TPS)48T/96TY10071Sus scrofa; Porcine (Pig)
抗鞘磷脂少突膠質(zhì)細(xì)胞糖蛋白抗體(Anti-MOG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Myelin Oligodendrocyte Glycoprotein Antibody (Anti-MOG)48T/96TY10072Homo sapiens (Human)
Ⅰ型膠原交聯(lián)羧基端肽(CTXⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cross Linked C-opeptide Of Type I Collagen (CTXI)48T/96TY10073Mus musculus (Mouse)
乳酸脫氫酶(LDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactate Dehydrogenase (LDH)48T/96TY10074Rattus norvegicus (Rat)
軸突生長誘向因子1(Ntn1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Netrin 1 (Ntn1)48T/96TY10075Rattus norvegicus (Rat)
白介素2受體α(IL2Rα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra)48T/96TY10076Rattus norvegicus (Rat)
凝集素樣氧化低密度脂蛋白受體1(LOX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1)48T/96TY10077Homo sapiens (Human)
β2-微球蛋白(β2M)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-2-Microglobulin (b2M)48T/96TY10078Rhesus monkey (Simian)
表皮生長因子受體(EGFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Epidermal Growth Factor Receptor (EGFR)48T/96TY10079Rattus norvegicus (Rat)
Ⅱ型膠原交聯(lián)羧基端肽(CTXⅡ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cross Linked C-opeptide Of Type II Collagen (CTXII)48T/96TY10080Mus musculus (Mouse)
胎球蛋白B(FETUB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fetuin B (FETUB)48T/96TY10081Homo sapiens (Human)
核因子κB(NFκB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Kappa B (NFkB)48T/96T Y10082Homo sapiens (Human)
Ⅰ類主要組織相容性復(fù)合體G(MHCG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Histocompatibility Complex Class I G (MHCG)48T/96TY10083Homo sapiens (Human)
肌鈣蛋白T(TNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin T (TNT)48T/96TY10084Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。